【文献标题】PM2.5 exposure induces reproductive injury through IRE1/JNK/autophagy signaling in male rats
【作者】Yang Yang，Yajing Feng，Hui Huang，et.al
【作者单位】郑州大学第一附属医院（The First Affiliated Hospital of Zhengzhou University）
【关键词】Fine particulate matter，Inositol-requiring enzyme，C-Jun NH 2-terminal kinase，Autophagy，Reproductive injury
【出版期刊】《Ecotoxicology and Environmental Safety》
2.5. Determination of serum testosterone
We evaluated serum testosterone level in rats by ELISA. After the rats were sacrificed, approximately 5 mL of abdominal aortic blood was collected and subjected to centrifugation at 3000 rpm for 15 min, and the serum was carefully aspirated and stored at 20 for later use. According to the instructions for the rat testosterone ELISA kit (Hengyuan Biotech, Shanghai, China), we used different concentrations of testosterone standards and corresponding absorbance values to prepare a standard curve, and thus determined the testosterone in each group of samples after observing the sample absorbance.